Technology Advances

New Chromogenic Multiplex IHC Kits for Rapid Manual Staining

February 21, 2022

Cell IDx announces the release of our new chromogenic multiplex IHC kits for manual staining of human FFPE tissue sections. This simple, rapid, out-of-the-box solution allows you to stain, image and analyze, all in one day, and is an excellent complement to our current rapid BOND RX-optimized Chromogenic Multiplex IHC Kits. We invite you to view our gallery and contact us at info@cellidx.com about custom chromogenic multiplex IHC panels!

Virtual Workshop: ZEISS Multiplex Imaging Solutions Workshop

February 8–10, 2022

Cell IDx participated in Zeiss’ workshop. Watch the presentation titled Multiplex Immunofluorescence and Multiplex Immunochromogenic Staining Employing Cell IDx’s UltraPlex Tag-based Technology and following Q&A from the workshop presented by David Schwartz, Cell IDx’s Co-Founder, Chief Executive Officer, and Chief Scientific Officer.

Cell IDx Announces the release of UltraPlex Mouse Fluorescent Multiplex IHC Panels

October 22, 2021

Announcing the release of Mouse Fluorescent Multiplex IHC staining panels for preclinical research, the newest member of our UltraPlex technology family! Our modular format easily allows customers to build their own multiplex panel by selecting from our portfolio of biomarkers and choosing their preferred set of fluors. Staining with UltraPlex technology either manually or on the autostainer is quick and easy, using a single antigen retrieval and two staining steps using cocktails of antibodies. Contact us at info@cellidx.com about additional novel biomarkers or custom chromogenic multiplex IHC panels! Read more

Simultaneous 6-color, 5-biomarker Multiplex Tissue Staining, with A Single Antigen Retrieval Step and No Spectral Unmixing!

June 21, 2021

Learn how UltraPlex technology with the inclusion of a megastokes dye can expand your biomarker profiling and enable rapid, high-throughput, 5-biomarker tissue staining and analysis using standard equipment, without the need for spectral unmixing. Download our UltraPlex poster to see how. Read poster.

Application of Chromogenic Multiplex IHC to Lung Cancer Biomarker Profiling

April 14, 2021

Please feel free to download the poster LB238, entitled “Multiplex immunohistochemistry profiling with UltraPlex IHC on FFPE lung cancer provides a fast, robust staining platform compatible with pathology laboratory workflows,” which was presented this month at AACR21 and demonstrates the easy application of the UltraPlex technology. Thank you for stopping by our virtual booth and we look forward to seeing you in May!

Did you know that Cell IDx services are now on scientist.com?

September 2, 2020

Cell IDx is a Registered Supplier with scientist.com, so sign in and send us a request today. Access our full-service multiplex biomarker tissue section staining, imaging, and analysis services using both fluorescent and chromogenic multiplex panels in human and in mouse.

Cell IDx is pleased to announce the co-authorship and use of our UltraPlex mxIF technology for profiling of the TME in this important study

March 6, 2020

A Phase I trial assessing the safety and tolerability of a therapeutic DNA vaccination against HPV16 and HPV18 E6/E7 oncogenes after chemoradiation for cervical cancer. View a summary of this paper by Hasan et al.

Nature: Single-cell Analysis Reveals New Evolutionary Complexity in Uveal Melanoma

January 24, 2020

Cell IDx is pleased to announce the co-authorship and inclusion of our UltraPlex mxIF technology in the University of Miami’s Nature Communications paper “Single-cell analysis reveals new evolutionary complexity in uveal melanoma.” Read the full paper on Nature’s website.

Cell IDx Webinar on “Immunofluorescence Profiling of Tumor Biopsies Using UltraPlex mxIF Technology”

April 6, 2018

Please join Helen Snyder, our Director of Preclinical Development & Strategic Partnerships for a joint Cell IDx/Leica webinar looking at the application of multiplex immunofluorescence for single slide, comprehensive analysis of immune infiltrates within tumor biopsies. Watch On Demand after April 6.

GEN Feature Articles
Supplement: Who Gets Immunotherapy for Cancer?

April 1, 2018

Single-agent or combination checkpoint inhibition (CPI) immunotherapy can have dramatic impacts for positive responders. But currently-used nonspecific biomarkers are unable to definitively stratify and identify cohorts. This is a critical challenge; no one wants to deny care. Targeted biomarkers and companion diagnostics can greatly aid therapeutic decision making. Immunotherapy applications require more than just quantification of infiltrating immune cell subpopulations. Context is crucial, and concentrated efforts are underway to provide accurate information on the percentage and location of infiltrating immune cell subpopulations, both relative to each other and to tumor margins, as well as levels of expression of checkpoint and other immune markers. The Cell IDx UltraPlex mxIF (multiplex immunofluorescence) technology, a simple two-hour staining procedure, can simultaneously detect multiple biomarkers in tissue using a standard two-step primary and secondary antibody protocol and existing autostainers, imaging scanners, and image-analysis software. The technology is modular (plug and play); approximately 30 primary antibodies have been qualified thus far. Read the full article from GEN.

Cell IDx Announces Publication of a Book Chapter Demonstrating the Use of UltraPlex Technology in ICC (Immunocytochemistry)

April 7, 2017

Cell IDx in collaboration with Bio-Techne announces the publication of a chapter titled “Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX Using Primary Antibodies Raised in the Same Host Species” in Signal Transduction Immunohistochemistry, Volume 1554 of Methods of Molecular Biology. (PUBMED). The work demonstrates the simultaneous detection of EGFR and H2AX in A431 human epidermoid carcinoma cells using Cell IDx’s proprietary hapten/high affinity anti-hapten antibody technology. This work further expands the utility of the Cell IDx UltraPlex technology for use in ICC. Further work expanding the number of biomarkers simultaneously detected in a single assay is planned.

UltraPlex technology 3–4X brighter than fluorescent primaries!

June 25, 2015

Cell IDx’s multiplex immunofluorescence, breakthrough technology has solved the problem that has been perplexing histologists for decades — the ability to detect multiple markers on a single tissue!

The left panel consists of directly labeled anti-ER (blue), PR (green) and HER2 (red) antibodies. The right panel consists of anti-ER (blue), PR (green) and HER2 (red) antibodies detected using UltraPlex reagents.

Cell IDx’s UltraPlex technology provides the freedom to create multiplex IHC panels with vivid staining and without antibody species restriction. See our Multiplex Gallery.

Have you ever imagined how different your research capabilities would be if you could get multiple marker, flow-cytometry quality data on cells while also identifying their localization in tissue sections? Cell IDx has the solution!

Multiplex IHC has, in the past, been mired in difficulties:

• Fluorescent-labeled primaries just don’t give the intensity of staining that you need for sensitive detection.
• The need to use fluorescent-labeled anti-species secondaries to detect your primary antibody limits the number of markers that can be analyzed at one time.
• Attempts to solve this problem have involved repeated rounds of staining and stripping that are time consuming and can affect levels of detection.

As shown above, UltraPlex technology provides the same flexibility to detect markers that you would get using primary antibodies but provides 3–4X brighter fluorescence.

Contact us for more information.

Comparison of Cell IDx UltraPolymer Secondary Antibody-HRP Polymers versus Competitors

March 28, 2015

High sensitivity and specificity are the hallmarks of any immunodetection reagent. The development of secondary antibody-HRP polymers was a major step forward to increase sensitivity and specificity of IHC immunoperoxidase-based staining. These reagents also reduced the number of steps and significantly shortened the time required to stain tissues. However commercially available secondary antibody-HRP polymers produce widely disparate images with respect to sensitivity and specificity.

Cell IDx introduces our UltraPolymer Secondary Antibody-HRP Polymers that produce images with equal or greater sensitivity and specificity compared to clinically used polymers. The image below compares Cell IDx anti-mouse and anti-rabbit secondary antibody-HRP polymers to both Dako and Biocare’s polymers. If you are interested in comparing our UltraPolymers to the HRP polymer you are currently using, please let us know.

We welcome any suggestions for other high sensitivity peroxidase reagents to evaluate by comparison. We want to prove that we offer the most sensitive, most specific, and most cost-effective peroxidase conjugates you can buy!

Staining of ER+ PR+ Ki-67+ breast carcinoma sections using anti-ER and anti-PR rabbit monoclonal antibodies and anti-Ki-67 mouse monoclonal antibody and detecting using either Cell-IDx’s UltraPolymer Secondary Antibody-HRP polymers or Biocare’s Mach4 or Dako’s Envision+ staining systems. Click for more detailed staining protocol and image.