Cell IDx

Make Your Own Fluorescent or Chromogenic Multiplex IHC Panel Using UltraTag Multiplex Labeling Kits

UltraTag Multiplex Labeling Kits allow you the freedom to transform your great IHC antibodies into fluorescent or chromogenic multiplex IHC staining panels! Choose the number of biomarkers/kits required and then rapidly transform your IHC antibodies from a single brown stain into 4-color fluorescent multiplex or 3-color chromogenic multiplex immunohistochemistry panels. UltraTag kits efficiently label 50µg @ 1.0 mg/mL of high-affinity, purified antibody in ~2 hours.

UltraPlex technology then allows simple, rapid tissue staining using cocktails of tagged-primary and fluor- or enzyme-labeled anti‑Tag secondary antibodies. You can go from staining to imaging to analysis in one day!

Advantages

Simple labeling procedure, high efficiency tagging, and >80% recovery.
Inclusive kit contains UltraTag labeling reagent and fluor- or enzyme-labeled anti‑Tag detector antibody.
Enables simultaneous detection of multiple primary antibodies from the same species in a single multiplex stain.

Fluorescent Multiplex Labeling Kits

Select 4 different Fluorescent UltraTag kits to create a 4‑plex fluorescent multiplex IHC panel.
Two 4-kit sets available for imagers with (CL490, 550, 595, and 650 fluors) and without spectral unmixing (CL490, 550, 650, and 750 fluors).
All components required to label 50µg of user-provided carrier-free antibody (e.g., no extraneous proteins such as BSA, gelatin, or serum) @ 1mg/mL with >80% yield, and sufficient fluor-labeled anti-Tag detector antibody to stain 15–20 slides. Additional anti‑tag detector antibody is available.
Only equipment required are a pipette and microcentrifuge.

Chromogenic Multiplex Labeling Kits

Antibodies labeled with different Chromogenic UltraTag kits can be combined to create 3‑plex chromogenic multiplex IHC panels using included enzyme-labeled anti‑Tag antibodies.
All components required to label 50µg of user-provided carrier-free antibody (e.g., no extraneous proteins such as BSA, gelatin, or serum) @ 1mg/mL with >80% yield, and sufficient enzyme-labeled anti‑tag detector antibody to stain 15–20 slides. Additional anti‑tag detector antibody is available.
Only equipment required are a pipette and microcentrifuge.
Components needed for staining but not supplied include chromogens and HRP-arrest solution.

How Do I Know If My Antibody Is A Good Candidate for Labeling with UltraTag Kits?

The 50µg antibody is at 0.9–1.1mg/mL in buffer free of extraneous proteins such as BSA, serum, or gelatin. Antibody formulations containing azide, trehalose, or other sugars, as well as, any buffer containing tris, are acceptable.
Select antibodies that work well in your desired application. We recommend selecting clones which stain strongly at <1µg/mL when tested by conventional IHC. However, each antibody is different, and performance of individual clones cannot be guaranteed.

Click on Catalog # to view detailed product information.

Product Name	Description	Size	Catalog #	List Price
UltraTag AP1 Kit	Kit to label 50 μg of purified antibody with UT015 Tag, and AP labeled anti-UT015 Tag antibody to stain 15-20 slides	1 Kit	UTCK-AP1	$395
UltraTag HRP1 Kit	Kit to label 50 μg of purified antibody with UT016 Tag, and HRP labeled anti-UT016 Tag antibody to stain 15-20 slides	1 Kit	UTCK-HRP1	$395
UltraTag HRP2 Kit	Kit to label 50 μg of purified antibody with UT021 Tag, and HRP labeled anti-UT021 Tag antibody to stain 15-20 slides	1 Kit	UTCK-HRP2	$395
UltraTag AP1 detection antibody	Supplementary AP labeled anti-UT015 Tag antibody for use with UltraTag kits	Stains 15-20 slides	UTCX-AP1	$295
UltraTag HRP1 detection antibody	Supplementary HRP labeled anti-UT016 Tag antibody	Stains 15-20 slides	UTCX-HRP1	$295
UltraTag HRP2 detection antibody	Supplementary HRP labeled anti-UT021 Tag antibody	Stains 15-20 slides	UTCX-HRP2	$295
UltraTag 490 Kit	Kit to label 50 μg of purified antibody with UT015 Tag, and CL490 fluor-labeled anti-UT015 Tag detector antibody to stain 15-20 slides	1 Kit	UTFK-490	$395
UltraTag 550 Kit	Kit to label 50 μg of purified antibody with UT016 Tag, and CL550 fluor-labeled anti-UT016 Tag detector antibody to stain 15-20 slides	1 Kit	UTFK-550	$395
UltraTag 595 Kit	Kit to label 50 μg of purified antibody with UT019 Tag, and CL595 fluor labeled anti-UT019 Tag detector antibody to stain 15-20 slides	1 Kit	UTFK-595	$395
UltraTag 650 Kit	Kit to label 50 μg of purified antibody with UT021 barcode, and CL650 fluor labeled anti-UT021 Tag detector antibody to stain 15-20 slides	1 Kit	UTFK-650	$395
UltraTag 750 Kit	Kit to label 50 μg of purified antibody with UT019 barcode, and CL750 fluor labeled anti-UT019 Tag detector antibody to stain 15-20 slides	1 Kit	UTFK-750	$395
UltraTag 490 detection antibody	Supplementary CL490 fluor labeled anti-UT015 Tag detector antibody	Stains 15-20 slides	UTFX-490	$295
UltraTag 550 detection antibody	Supplementary CL550 fluor labeled anti-UT016 Tag detector antibody	Stains 15-20 slides	UTFX-550	$295
UltraTag 595 detection antibody	Supplementary CL595 fluor labeled anti-UT019 Tag detector antibody	Stains 15-20 slides	UTFX-595	$295
UltraTag 650 detection antibody	Supplementary CL650 fluor labeled anti-UT021 Tag detector antibody	Stains 15-20 slides	UTFX-650	$295
UltraTag 750 detection antibody	Supplementary CL750 fluor labeled anti-UT019 Tag detector antibody	Stains 15-20 slides	UTFX-750	$295

Resources

For Research Use Only. Not for use in diagnostic procedures.

UltraTag Multiplex Labeling Kits

Make Your Own Fluorescent or Chromogenic Multiplex IHC Panel Using UltraTag Multiplex Labeling Kits

Advantages

Fluorescent Multiplex Labeling Kits

Chromogenic Multiplex Labeling Kits

How Do I Know If My Antibody Is A Good Candidate for Labeling with UltraTag Kits?

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Resources

Chromogenic Protocols

Fluorescent Protocols

References

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