For the First Time — Multiplex Any 4 Antibodies!Conjugate Up to 4 Antibodies for Multiplex IHC Experiments
Make your own multiplex IHC panel in around 2 hours with UltraTag Multiplex Labeling Kits. Choose the number of biomarkers/kits you need and rapidly transform your IHC antibodies from a single brown stain to 2-, 3-, and 4-color fluorescent multiplex immunohistochemistry panels. Allows simple, rapid, two-step staining using cocktails of primary and secondary antibodies. Now you can go from labeling to multiplex immunofluorescence tissue staining, imaging, and biomarker analysis in less than a day!
UltraTag kits efficiently label 50µg of purified antibody in 50µL with one of four available hapten tags in around two hours, with ~100% tagging and >80% recovery. Up to four primary antibodies tagged with four different hapten-tagged kits can be used together and detected independently. The kit includes fluor-labeled anti-hapten detector antibody sufficient to stain 15–20 slides. Additional anti-hapten detector antibody available. Compatible with biotin or digoxygenin detection, to allow independent analysis of up to six antigens.
Scheme of UltraTag multiplex immunofluorescence (mxIF) staining.
Multiplex image of tonsil tissue stained with hapten-labeled
Advantages
- Simple labeling procedure, 100% tagging, 20 minutes hands-on time.
- Inclusive kit containing hapten labeling reagent and detector antibody.
- Choice of four-hapten/fluor-labeled anti-hapten antibody pairs to allow multiplex immunoassays
- Multiplex staining using cocktails of primary and secondary antibodies in a simple two-step, two-hour staining protocol
- Enable detection of multiple antibodies from the same species in one multiplex experiment.
Applications
- Easily identify cell sub-populations expressing multiple biomarkers in situ in sections.
- Application to immune profiling of the tumor TME microenvironment for immuno-oncology.
- Follow expression of pathway markers.
- 2-, 3-, or 4-color multiplex immunohistochemistry staining of tissue sections or cell spreads.
- Overcomes mouse-on-mouse restrictions in xenograft models.
- Platform technology which can be used in other immunoassays, e.g., ICC.
UltraTag Multiplex Labeling Kits & Complementary Products
- Each kit conjugates a hapten to 50µg @ 1.0µg/µL of carrier-free, (e.g., BSA) antibody with >80% yield, and fluor-labeled anti-hapten detector antibody to stain 15–20 slides.
- Only equipment required are a pipette and microcentrifuge.
- Also available (see Product Listing below)
- Additional fluor-labeled anti-hapten antibody
- Custom conjugation of detection modalities to anti-hapten antibodies
- Three haptens available with CL490, CL550, and CL650 fluor-labeled anti-hapten antibodies.
- Fourth hapten available with both CL594 and CL750 fluors to allow easy multiplex detection using a variety of scanners and microscopes.
How do I know if my antibody is a good candidate for labeling with UltraTag Kits?
- The antibody is protein carrier free, i.e., no gelatin, BSA, serum, etc. Antibody formulations are acceptable that contain azide, trehalose, or other sugars, as well as any buffer containing tris.
- Has a confirmed concentration of 0.9–1.1mg/mL and amount of 50µg.
- Has been tested and works well in my desired assay, e.g., at 1µg/mL or less for immunohistochemistry.
Resources
- Download: Protocol (pdf)
- Download: Absorption & Emission of CellLight Fluors Conjugated to Anti-Hapten Detector Antibodies (pdf)
Contact us at info@cellidx.com for custom services to conjugate your detection reagent to anti-hapten antibodies.
Click on Catalog # to view detailed product information.
| Product Name | Description | Size | Catalog # | List Price | Quantity | Add to Cart |
|---|---|---|---|---|---|---|
| UltraTag AP1 Kit | Kit to label 50 μg of purified antibody with UT015 Tag, and AP labeled anti-UT015 Tag antibody to stain 15-20 slides | 1 Kit | UTCK-AP1 | $395 | ||
| UltraTag HRP1 Kit | Kit to label 50 μg of purified antibody with UT016 Tag, and HRP labeled anti-UT016 Tag antibody to stain 15-20 slides | 1 Kit | UTCK-HRP1 | $395 | ||
| UltraTag HRP2 Kit | Kit to label 50 μg of purified antibody with UT021 Tag, and HRP labeled anti-UT021 Tag antibody to stain 15-20 slides | 1 Kit | UTCK-HRP2 | $395 | ||
| UltraTag AP1 detection antibody | Supplementary AP labeled anti-UT015 Tag antibody | Stains 15-20 slides | UTCX-AP1 | $295 | ||
| UltraTag HRP1 detection antibody | Supplementary HRP labeled anti-UT016 Tag antibody | Stains 15-20 slides | UTCX-HRP1 | $295 | ||
| UltraTag HRP2 detection antibody | Supplementary HRP labeled anti-UT021 Tag antibody | Stains 15-20 slides | UTCX-HRP2 | $295 | ||
| UltraTag 490 Kit | Kit to label 50 μg of purified antibody with UT015 Tag, and CL490 fluor labeled anti-UT015 Tag detector antibody to stain 15-20 slides | 1 Kit | UTFK-490 | $395 | ||
| UltraTag 550 Kit | Kit to label 50 μg of purified antibody with UT016 Tag, and CL550 fluor labeled anti-UT016 Tag detector antibody to stain 15-20 slides | 1 Kit | UTFK-550 | $395 | ||
| UltraTag 595 Kit | Kit to label 50 μg of purified antibody with UT019 Tag, and CL595 fluor labeled anti-UT019 Tag detector antibody to stain 15-20 slides | 1 Kit | UTFK-595 | $395 | ||
| UltraTag 650 Kit | Kit to label 50 μg of purified antibody with UT021 barcode, and CL650 fluor labeled anti-UT021 Tag detector antibody to stain 15-20 slides | 1 Kit | UTFK-650 | $395 | ||
| UltraTag 750 Kit | Kit to label 50 μg of purified antibody with UT019 barcode, and CL750 fluor labeled anti-UT019 Tag detector antibody to stain 15-20 slides | 1 Kit | UTFK-750 | $395 | ||
| UltraTag 490 detection antibody | Supplementary CL490 fluor labeled anti-UT015 Tag detector antibody | Stains 15-20 slides | UTFX-490 | $295 | ||
| UltraTag 550 detection antibody | Supplementary CL550 fluor labeled anti-UT016 Tag detector antibody | Stains 15-20 slides | UTFX-550 | $295 | ||
| UltraTag 595 detection antibody | Supplementary CL595 fluor labeled anti-UT019 Tag detector antibody | Stains 15-20 slides | UTFX-595 | $295 | ||
| UltraTag 650 detection antibody | Supplementary CL650 fluor labeled anti-UT021 Tag detector antibody | Stains 15-20 slides | UTFX-650 | $295 | ||
| UltraTag 750 detection antibody | Supplementary CL750 fluor labeled anti-UT019 Tag detector antibody | Stains 15-20 slides | UTFX-750 | $295 |
- For Research Use Only. Not for use in diagnostic procedures.
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