The UltraTag Hapten Labeling Kits efficiently conjugate 50 µg of antibody in 50 µL with one of four available hapten tags in less than an hour, with ~100% tagging, >80% recovery, and with less than two-fold dilution. Then, the tagged antibody can be used in any immunoassay and subsequently detected by the corresponding anti-hapten antibody. Up to four antibodies tagged with the four different haptens can be used together and detected independently. Does not interfere with other methods, such as conventional detection, and is compatible with biotin or digoxigenin detection, to allow independent analysis of up to six antigens.

Applications

  • Independently detect multiple monoclonal and/or polyclonal primary antibodies from the same or different species in one experiment
  • 2, 3, or 4 color immunofluorescence on tissues, cells, spheroids, or organoids
  • 2 or 3 chromogen immunohistochemistry on cell spreads or tissue sections
  • Use in other immunoassays, e.g., EliSpot, flow cytometry, ELISA

Characteristics

  • Standard, two step staining protocols are used, applying the hapten-labeled primary antibodies and then the fluor-labeled anti-hapten secondary antibodies as cocktails
  • Four-hapten/fluor-labeled anti-hapten antibody pairs available for multiplexing
  • Overcomes primary antibody species limitations
  • Overcomes species-on-species cross-reactivity
  • Sensitivity for each primary antibody remains nearly the same as for conventional anti-species secondary antibody detection
  • Allows mixing-and-matching of fluors or enzymes for any antibody

Figure 1. Scheme of UltraTag mxIF multiplex immune-fluorescence staining.

Figure 2. Multiplex image of tonsil tissue stained with hapten-labeled CD8 (green), CD4 (purple), PD-1 (red), and FoxP3 (yellow) prepared using the Cell IDx UltraTag Hapten Labeling Kit.

UltraTag Hapten Labeling Kits & Complementary Products

  • Each kit conjugates a hapten to 50 µg @ 1.0 µg/µL of carrier-free (e.g., BSA) antibody in >80% yield and fluor-labeled anti-hapten antibody to stain 15 slides
  • Only equipment required are a pipette and microcentrifuge
  • Also available (see Product Listing below)
    • Additional fluor-labeled anti-hapten antibody
    • HRP and AlkPhos conjugated anti-hapten antibodies
    • Custom conjugation of detection modalities to anti-hapten antibodies
  • Fourth fluor-labeled anti-hapten antibody available modified conjugated to CL595 or CL750

Figure 3. Example of product packaging.

Absorption & Emission of CL Dyes Conjugated to Fluor-labeled Anti-hapten Secondary Antibodies

Catalog # Hapten Fluor Absorbance
(nm)
Emission
(nm)
Extinction Coefficient
(M-1cm-1)
UTK-490 CH015 CL490 491 515 73,000
UTK-550 CH014 CL550 550 575 150,000
UTK-595 CH019 CL595 594 615 92,000
UTK-650 CH021 CL650 655 676 250,000
UTK-750 CH019 CL750 759 780 240,000

How do I know if my antibody is a good candidate for labeling with UltraTag Kits?

  • The antibody is protein carrier free, i.e., no gelatin, BSA, serum, etc. Antibody formulations are acceptable that contain azide, trehalose, or other sugars, as well as any buffer containing tris.
  • There is 50µg of antibody with a confirmed concentration of 0.9–1.1mg/mL.
  • The antibody has been tested and works well in the desired assay, e.g., at 1µg/mL or less for immunohistochemistry.

Contact us at info@cellidx.com for custom services to conjugate your detection reagent to anti-hapten antibodies.

Resources

Click on Catalog # to view detailed product information.

Product Name Description Size Catalog # List Price Quantity Add to Cart
UltraTag Kit 490 Kit to label 50 μg of purified antibody with CH015 hapten, and CL490 fluor labeled anti-CH015 detector antibody to stain 15-20 slides 1 Kit UTK-490 $395
UltraTag Kit 550 Kit to label 50 μg of purified antibody with CH014 hapten, and CL550 fluor labeled anti-CH014 detector antibody to stain 15-20 slides 1 Kit UTK-550 $395
UltraTag Kit 595 Kit to label 50 μg of purified antibody with CH019 hapten, and CL595 fluor labeled anti-CH019 detector antibody to stain 15-20 slides 1 Kit UTK-595 $395
UltraTag Kit 650 Kit to label 50 μg of purified antibody with CH021 hapten, and CL650 fluor labeled anti-CH021 detector antibody to stain 15-20 slides 1 Kit UTK-650 $395
UltraTag Kit 750 Kit to label 50 μg of purified antibody with CH019 hapten, and CL750 fluor labeled anti-CH019 detector antibody to stain 15-20 slides 1 Kit UTK-750 $395
anti-CH015-CL490 Supplementary CL490 fluor labeled anti-CH015 detector antibody Stains 15-20 slides UUH-490 $295
anti-CH014-CL550 Supplementary CL550 fluor labeled anti-CH014 detector antibody Stains 15-20 slides UUH-550 $295
anti-CH019-CL595 Supplementary CL595 fluor labeled anti-CH019 detector antibody Stains 15-20 slides UUH-595 $295
anti-CH021-CL650 Supplementary CL650 fluor labeled anti-CH021 detector antibody Stains 15-20 slides UUH-650 $295
anti-CH019-CL750 Supplementary CL750 fluor labeled anti-CH019 detector antibody Stains 15-20 slides UUH-750 $295
anti-CH014-HRP anti-CH014-horseradish peroxidase conjugate 15 μg YEH-001 $295
anti-CH019-HRP anti-CH019-horseradish peroxidase conjugate 15 μg YEH-002 $295
anti-CH015-alkaline phosphatase anti-CH015-alkaline phosphatase conjugate 15 μg YEK-001 $295
  • For Research Use Only. Not for use in diagnostic procedures.

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