Conjugate Up to 4 Antibodies for Multiplex IHC Experiments
Make your own multiplex IHC panel in around 2 hours with UltraTag Multiplex Labeling Kits. Choose the number of biomarkers/kits you need and rapidly transform your IHC antibodies from a single brown stain to 2-, 3-, and 4-color fluorescent multiplex immunohistochemistry panels. Allows simple, rapid, two-step staining using cocktails of primary and secondary antibodies. Now you can go from labeling to multiplex immunofluorescence tissue staining, imaging, and biomarker analysis in less than a day!
UltraTag kits efficiently label 50µg of purified antibody in 50µL with one of four available hapten tags in around two hours, with ~100% tagging and >80% recovery. Up to four primary antibodies tagged with four different hapten-tagged kits can be used together and detected independently. The kit includes fluor-labeled anti-hapten detector antibody sufficient to stain 15–20 slides. Additional anti-hapten detector antibody available. Compatible with biotin or digoxygenin detection, to allow independent analysis of up to six antigens.
Figure 1. Scheme of UltraTag multiplex immunofluorescence (mxIF) staining.
Figure 2. Multiplex image of tonsil tissue stained with hapten-labeled CD8 (green), CD4 (purple), PD-1 (red), and FoxP3 (yellow) prepared using the prepared using UltraTag Multiplex Labeling Kits.
Advantages
- Simple labeling procedure, 100% tagging, 20 minutes hands-on time.
- Inclusive kit containing hapten labeling reagent and detector antibody.
- Choice of four-hapten/fluor-labeled anti-hapten antibody pairs to allow multiplex immunoassays
- Multiplex staining using cocktails of primary and secondary antibodies in a simple two-step, two-hour staining protocol
- Enable detection of multiple antibodies from the same species in one multiplex experiment.
Applications
- Easily identify cell sub-populations expressing multiple biomarkers in situ in sections.
- Application to immune profiling of the tumor microenvironment (TME) for immuno-oncology.
- Follow expression of pathway markers.
- 2-, 3-, or 4-color multiplex immunohistochemistry staining of tissue sections or cell spreads.
- Overcomes mouse-on-mouse restrictions in xenograft models.
- Platform technology which can be used in other immunoassays, e.g., ICC.
UltraTag Multiplex Labeling Kits & Complementary Products
- Each kit conjugates a hapten to 50µg @ 1.0µg/µL of carrier-free (e.g., BSA) antibody with >80% yield, and fluor-labeled anti-hapten detector antibody to stain 15–20 slides.
- Only equipment required are a pipette and microcentrifuge.
- Also available (see Product Listing below)
- Additional fluor-labeled anti-hapten antibody
- Custom conjugation of detection modalities to anti-hapten antibodies
- Three haptens available with CL490, CL550, and CL650 fluor-labeled anti-hapten antibodies.
- Fourth hapten available with both CL594 and CL750 fluors to allow easy multiplex detection using a variety of scanners and microscopes.
Figure 3. Example of product packaging.
Absorption & Emission of CellLight Fluors Conjugated to Anti-hapten Detector Antibodies
| Catalog # | Hapten | Fluor | Absorbance (nm) |
Emission (nm) |
Extinction Coefficient (M-1cm-1) |
|---|---|---|---|---|---|
| UTK-490 | CH015 | CL490 | 491 | 515 | 73,000 |
| UTK-550 | CH014 | CL550 | 550 | 575 | 150,000 |
| UTK-595 | CH019 | CL595 | 594 | 615 | 92,000 |
| UTK-650 | CH021 | CL650 | 655 | 676 | 250,000 |
| UTK-750 | CH019 | CL750 | 759 | 780 | 240,000 |
How do I know if my antibody is a good candidate for labeling with UltraTag Kits?
- The antibody is protein carrier free, i.e., no gelatin, BSA, serum, etc. Antibody formulations are acceptable that contain azide, trehalose, or other sugars, as well as any buffer containing tris.
- Has a confirmed concentration of 0.9–1.1mg/mL and amount of 50µg.
- Has been tested and works well in my desired assay, e.g., at 1µg/mL or less for immunohistochemistry.
Resources
- Download: Protocol (pdf)
Contact us at info@cellidx.com for custom services to conjugate your detection reagent to anti-hapten antibodies.
Click on Catalog # to view detailed product information. If there are additional sizes available, click Size to expand for more size options; click again to close.
| Product Name | Description | Size | Catalog # | List Price | Quantity | Add to Cart |
|---|---|---|---|---|---|---|
| UltraTag Kit 490 | Kit to label 50 μg of purified antibody with CH015 hapten, and CL490 fluor labeled anti-CH015 detector antibody to stain 15-20 slides | 1 Kit | UTK-490 | $395 | ||
| UltraTag Kit 550 | Kit to label 50 μg of purified antibody with CH014 hapten, and CL550 fluor labeled anti-CH014 detector antibody to stain 15-20 slides | 1 Kit | UTK-550 | $395 | ||
| UltraTag Kit 595 | Kit to label 50 μg of purified antibody with CH019 hapten, and CL595 fluor labeled anti-CH019 detector antibody to stain 15-20 slides | 1 Kit | UTK-595 | $395 | ||
| UltraTag Kit 650 | Kit to label 50 μg of purified antibody with CH021 hapten, and CL650 fluor labeled anti-CH021 detector antibody to stain 15-20 slides | 1 Kit | UTK-650 | $395 | ||
| UltraTag Kit 750 | Kit to label 50 μg of purified antibody with CH019 hapten, and CL750 fluor labeled anti-CH019 detector antibody to stain 15-20 slides | 1 Kit | UTK-750 | $395 | ||
| anti-CH015-CL490 | Supplementary CL490 fluor labeled anti-CH015 detector antibody | Stains 15-20 slides | UUH-490 | $295 | ||
| anti-CH014-CL550 | Supplementary CL550 fluor labeled anti-CH014 detector antibody | Stains 15-20 slides | UUH-550 | $295 | ||
| anti-CH019-CL595 | Supplementary CL595 fluor labeled anti-CH019 detector antibody | Stains 15-20 slides | UUH-595 | $295 | ||
| anti-CH021-CL650 | Supplementary CL650 fluor labeled anti-CH021 detector antibody | Stains 15-20 slides | UUH-650 | $295 | ||
| anti-CH019-CL750 | Supplementary CL750 fluor labeled anti-CH019 detector antibody | Stains 15-20 slides | UUH-750 | $295 | ||
| anti-CH014-HRP | anti-CH014-horseradish peroxidase conjugate | 15 μg | YEH-001 | $295 | ||
| anti-CH019-HRP | anti-CH019-horseradish peroxidase conjugate | 15 μg | YEH-002 | $295 | ||
| anti-CH015-alkaline phosphatase | anti-CH015-alkaline phosphatase conjugate | 15 μg | YEK-001 | $295 |
- For Research Use Only. Not for use in diagnostic procedures.