Allows Detection of Multiple Markers Using 1° Antibodies from Any Species
High definition chromogenic multiplex IHC staining using UltraPlex technology allows precise visualization of tissue biomarkers. Our rapid staining technology and simple workflow using cocktails of antibodies and a single antigen retrieval step
allows you to go from staining to analysis in a single day. Our range of BOND RX/RXm autostainer-optimized staining panels provides a seamless, high-throughput solution for biomarker tissue profiling.
Routine multiplex immunohistochemistry detection has been limited to two chromogens using primary antibodies from different species detected using enzyme-labeled secondary anti-species antibodies followed by chromogen precipitation. Cell IDx’s UltraPlex hapten technology
overcomes many of these limitations allowing detection of 3–4 markers using primary antibodies from any species while employing a single antigen retrieval step on an autostainer.
Introducing UltraPlex Chromogenic Multiplex IHC
Autostainer-Optimized Staining Panels for BOND RX/RXm
Chromogenic Multiplex Staining of PD-L1/CD8/PanCK
UltraPlex multiplex IHC staining of PD-L1/CD8/PanCK
panel on tonsil. Note: PD-L1 co-localized with PanCK produces a dark green color.
Chromogenic Multiplex Staining of CD4/CD8/PanCK
UltraPlex multiplex IHC staining of CD4/CD8/PanCK on colorectal cancer tissue .
Chromogenic Multiplex Staining of CD163/CD68/PD-L1
UltraPlex multiplex IHC staining of CD163/CD68/PD-L1 on lung adenocarcinoma.
Above images from an Aperio GT450 scanner.
To demonstrate that the UltraPlex IHC and UltraPlex IF technologies are detecting the same biomarkers, two serial tonsil slides were incubated with the same hapten-labeled primary cocktail and one slide was developed chromogenically and the other fluorescently using their
respectively-labeled anti-hapten antibodies. The results are presented below using the PD-L1/CD8/GranzymeB panel.
Chromogenic Multiplex Staining of PD-L1/CD8/GranzymeB
Multiplex IHC and IF images on serial section of tonsil with the PD-L1/CD8/GranzymeB three-plex as well as H&E staining of multiplex IF slide following removal of its coverslip.
The UltraPlex multiplex IHC protocol requires 4.5 hours for 3-marker staining, as illustrated below. The initial step in this protocol is identical to Cell IDx’s multiplex
immunofluorescence protocol in which a cocktail of hapten-labeled primary antibodies are incubated on the tissue. For chromogen detection, enzyme-labeled anti-hapten antibodies are employed instead of fluor-labeled anti-hapten antibodies. See protocol schema in