RSGO490-002 | ReadySetGo, Biotin/Streptavidin-CL490 Antibody Labeling & Detection Kit
All-in-one ultra rapid kit it to optimally label 50-100 µg of purified antibody with RightON biotin reagent, and CL490 Fluor labeled streptavidin detector reagent to stain 100 slides by IHC
All components required to label 50-100 µg of purified antibody with a recommended starting concentration 0.5-1.1 mg/ml in carrier-free buffer with biotin. The kit utilizes RightON technology to conjugate your antibody to biotin in 10 minutes with minimal hands-on time. It includes RightON biotin, a recommended red desalting column to eliminate free biotin from your assays, a modifier, a quencher, streptavidin-CL490 reagent sufficient to stain 100 slides by IHC.
Antibody needs to be at a concentration of 0.5-1.1mg/ml in buffer free of extraneous proteins such as BSA, serum or gelatin. Antibody formulations containing azide, trehalose or other sugars are acceptable. No tris buffer.
Biotin tube @ -20°C, other components @ 2-8°C
For In vitro Research Use Only. Not for diagnostic or therapeutic use.
Human FFPE tonsil section stained with 2ug/ml anti-PanCK labeled with ReadySetGo kit and detection with included streptavidin-CL490 at 2ug/ml