HI06A-005 | HI-6A Multiplex Panel - Human CD3, CD4, CD8, FoxP3
Kit for fluorescent multiplex IHC staining of human CD3, CD4, CD8, FoxP3, detection with 490, 550, 650, 750 nm fluors
All components required to stain 5 multiplex slides plus a single negative control slide with simple two-step protocol.<br>
<table> 1° Antibody| Clone| 1° Tag| 2° Antibody| 2° Fluor! CD3| EP4426| UT016| Anti-UT016| CL 650! CD4| EPR6855| UT019| Anti-UT019| CL 750! CD8| EPR10640(2)| UT015| Anti-UT015| CL 490! FoxP3| 1054C| UT014| Anti-UT014| CL 550! </table>
Optimized for use on FFPE tissue sections.<br>
Suitable for conventional imaging using defined filter sets <br>
Additional negative control (secondary reagents alone) are available, see NEG01A. <br>
Depending on your imaging scanner or microscope you may want to use Product HI06B with 490, 550, 594 and 650 nm fluors instead
Concentrated 1° antibody-Tag (0.5mg/ml) in PBS/15mM azide <br>
Concentrated 2° antibody-fluor (0.5mg/ml) in PBS/15mM azide <br>
Antibody diluent solution - PBS/1% BSA/15mM azide <br>
Tissue block solution - 3% rabbit serum in PBS/1%BSA/15mM azide <br>
For Research Use Only. Not for Diagnostic or Therapeutic Use. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Cell IDx, Inc. Product may not be resold or modified for resale without prior written approval of Cell IDx, Inc.
Anaplastic large cell lymphoma biopsy – lymph node - stained manually with HI-6A UltraPlex panel: CD3 (red), FoxP3 (yellow), CD8 (green), CD4 (purple). 20X magnification.
Endometriod adenocarcinoma biopsy - stained manually with HI-6A UltraPlex panel: CD3 (red), FoxP3 (yellow), CD8 (green), CD4 (purple). 20X magnification.