HI02A-005 | HI-2A mxIF Panel - CD68, CD163, Pan-CK, PD-L1
490, 550, 650, 750 nm fluors
All components required to stain 5 multiplex slides plus a single negative control slide with simple two-step protocol.<br>
<table> 1° Antibody| Clone| 1° Hapten| 2° Antibody| 2° Fluor! CD68| C68/684| CH019| Anti-CH019| CL 750! CD163| EPR19518*| CH015| Anti-CH015| CL 490! Pan-CK| AE1/AE3| CH016| Anti-CH016| CL 650! PD-L1| CAL10**| CH014| Anti-CH014| CL 550! </table>
Optimized for use on FFPE tissue sections.<br>
Suitable for conventional imaging using defined filter sets <br>
Additional negative control (secondary reagents alone) are available, see NEG01A. <br>
Depending on your imaging scanner or microscope you may want to use Product HI02B with 490, 550, 594 and 650 nm fluors instead
Concentrated 1° antibody-hapten (0.5mg/ml) in PBS/15mM azide <br>
Concentrated 2° antibody-fluor (0.5mg/ml) in PBS/15mM azide <br>
Antibody diluent solution - PBS/1% BSA/15mM azide <br>
Tissue block solution - 3% rabbit serum in PBS/1%BSA/15mM azide <br>
For Research Use Only. Not for Diagnostic or Therapeutic Use.
Lung cancer tissue stained for CD68 (purple), CD163 (green), Pan-CK (red) and PD-L1 (yellow). Tissue courtesy of Dr Jair Bar, Sheba Medical Institute.