2RH-1000 | Anti-Rabbit UltraPolymer HRP

Description
Goat anti-Rabbit IgG (H&L) - HRP - IHC & autostainer optimized
Specification
Affinity purified goat anti-rabbit IgG (H&L) conjugated to HRP, min x w/human, mouse or rat serum
Price
Catalog No.
2RH-1000
Size
1000mL
Form
Liquid, prediluted
Conjugate
HRP
Application
IHC-P - optimized for use with autostainer or manual staining
Buffer
TBS, 1% BSA, 0.1% proclin 150, 10% heat-inactivated animal serum
Purification
Affinity purified using solid phase Rabbit IgG
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Host
Goat
Clonality
Polyclonal
Immunogen
Purified Rabbit IgG, whole molecule
Storage
2-8°C
Shelf-Life
1 year
Specificity
Based on IEP, this immunopolymer antibody reacts with heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulins
Cross-Reactivity
Based on IEP, no cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat
Country of Origin
Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian
Disclaimer
For In vitro Research Use Only. Not for diagnostic or therapeutic use.

Tonsil stained with rabbit anti-human CD4 (clone EPR6855, 0.4ug/ml) and detected with UltraPolymer goat anti-rabbit-HRP (RTU). Staining on the BOND RX autostainer. Antigen retrieval, 20 min with ER2. Primary antibody, 30 min incubation. Secondary antibody, 15 min incubation. 20X magnification.

Lung cancer stained with rabbit anti-human CD4 (clone EPR6855, 0.4ug/ml) and detected with UltraPolymer goat anti-rabbit-HRP (RTU). Staining on the BOND RX autostainer. Antigen retrieval, 20 min with ER2. Primary antibody, 30 min incubation. Secondary antibody, 15 min incubation. 20X magnification.

Colon cancer stained with rabbit anti-human CD4 (clone EPR6855, 0.4ug/ml) and detected with UltraPolymer goat anti-rabbit-HRP (RTU). Staining on the BOND RX autostainer. Antigen retrieval, 20 min with ER2. Primary antibody, 30 min incubation. Secondary antibody, 15 min incubation. 20X magnification.

Tonsil stained with rabbit anti-human CD3 (clone EP4426, 1ug/ml) and detected with UltraPolymer goat anti-mouse-HRP (RTU). Manual staining. Antigen retrieval, 15 min high pressure (~120C) in pressure cooker using citrate, pH 6.0 buffer. Primary antibody, 60 min incubation. Secondary antibody, 30 min incubation. 20X magnification.

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