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Tonsil stained with mouse anti-human Ki-67 (clone B56, 0.4ug/ml) and detected with UltraPolymer goat anti-mouse-HRP (RTU). Staining on the BOND RX autostainer. Antigen retrieval, 20 min with ER2. Primary antibody, 30 min incubation. Secondary antibody, 15 min incubation. 20X magnification.
Lung cancer stained with mouse anti-human Ki-67 (clone B56, 0.4ug/ml) and detected with UltraPolymer goat anti-mouse-HRP (RTU). Staining on the BOND RX autostainer. Antigen retrieval, 20 min with ER2. Primary antibody, 30 min incubation. Secondary antibody, 15 min incubation. 20X magnification.
Colon cancer stained with mouse anti-human Ki-67 (clone B56, 0.4ug/ml) and detected with UltraPolymer goat anti-mouse-HRP (RTU). Staining on the BOND RX autostainer. Antigen retrieval, 20 min with ER2. Primary antibody, 30 min incubation. Secondary antibody, 15 min incubation. 20X magnification.
Tonsil stained with mouse anti-human Ki-67 (clone B56, 1ug/ml) and detected with UltraPolymer goat anti-mouse-HRP (RTU). Manual staining. Antigen retrieval, 15 min high pressure (~120C) in pressure cooker using citrate, pH 6.0 buffer. Primary antibody, 60 min incubation. Secondary antibody, 30 min incubation. 20X magnification.