Introducing UltraPlex Multiplexing Technology

UltraPlex mxIF (multiplex immunofluorescence) is a paradigm-shifting technology that addresses the needs of precision medicine for multiplex detection of proteins on tissues.

Cell IDx has developed a proprietary, modified hapten-based technology to address the need for unrestricted, simple, rapid, and simultaneous detection of multiple cellular markers in tissue sections.

Chromogenic detection of single markers can no longer keep pace with the requirement for detailed information on cell subpopulations, levels of marker expression per cell, and individual cellular localization in situ relative to other cells. To address these issues, we have developed this new technology with inspiration from flow-cytometry’s ability to phenotype and quantitate small subpopulations of cells identified by simultaneous expression of several markers, and translated this multiplex staining to tissue sections.

 

Hapten-based Staining Schema

Section Staining Interactive Tool

Click on each marker button to toggle marker on/off to visualize simultaneous expression of up to four markers.

Our Solution

Directly-labeled antibodies, such as are used in flow-cytometry, generally give weak staining in tissue sections compared to use of fluorescent-labeled secondary antibodies. However, use of fluorescent secondaries is limited by the availability of primary antibodies of different species or isotypes to avoid cross-reactivity. Cell IDx has developed an expanding range of next-generation, modified haptens which we use to label primary antibodies. Primary antibodies are combined in cocktails of four antibodies and then detected with a panel of anti-hapten secondary antibodies each labeled with a different fluor. The result is a simple two-hour, two-step staining procedure yielding the type of data previously impossible.

Key Benefits

  • Multiplex: Ability to detect >4 biomarkers plus DAPI on a single tissue specimen
  • Superplex: Tissue alignment of serial sections with different four-plex panels allows detection of 8, 12, or 16 markers provides an unsurpassed capacity for in situ cellular analysis
  • Antibody Species Independent: Mix and match your primary antibodies
  • Standard IHC Protocols Employed: Antigen retrieval followed by a two-hour, two-step staining protocol using cocktails of antibodies
  • Significant Time Savings: Stain in two hours (one-hour primary cocktail incubation followed by one-hour secondary cocktail incubation)
  • Autostainer, high-throughput compatible
  • Ability to detect multiple co-localized targets
  • Minimal tissue required: Core biopsies and TMAs

Order from our range of available products listed on this website or contact us to create a custom RUO multiplex assay panel for you.

See more images in the UltraPlex Gallery.

 

Head & Neck Squamous Cell Carcinoma: Pan-CK, PD-L1, CD68, CD163

Head & Neck Squamous Cell Carcinoma: CD4, CD8, CD20, FoxP3

Tissue alignment of the two above sections.

 

Luminal B Breast Cancer: HER2, ER, PR

Luminal B Breast Cancer: HER2, ER, PR, Ki-67

Head & Neck Squamous Cell Carcinoma: p16, CK5, EGFR, Vimentin

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